Overview of SCDE
scde package implements a set of statistical methods for analyzing single-cell RNA-seq data.
scde fits individual error models for single-cell RNA-seq measurements. These models can then be used for assessment of differential expression between groups of cells, as well as other types of analysis. The
scde package also contains the
pagoda framework which applies pathway and gene set overdispersion analysis to identify aspects of transcriptional heterogeneity among single cells.
The overall approach to the differential expression analysis is detailed in the following publication:
"Bayesian approach to single-cell differential expression analysis" (Kharchenko PV, Silberstein L, Scadden DT, Nature Methods, doi:10.1038/nmeth.2967)
The overall approach to pathways and gene set overdispersion analysis is detailed in the following publication: "Characterizing transcriptional heterogeneity through pathway and gene set overdispersion analysis" (Fan J, Salathia N, Liu R, Kaeser G, Yung Y, Herman J, Kaper F, Fan JB, Zhang K, Chun J, and Kharchenko PV, Nature Methods, doi:10.1038/nmeth.3734)
For additional installation information, tutorials, and more, please visit the SCDE website ☞
Sample analyses and images
Single cell error modeling
Differential expression analysis
lb mle ub ce Z cZ Dppa5a 8.075 9.965 11.541 8.075 7.160 5.968 Pou5f1 5.357 7.208 9.178 5.357 7.160 5.968 Gm13242 5.672 7.681 9.768 5.672 7.159 5.968 Tdh 5.829 8.075 10.281 5.829 7.159 5.968 Ift46 5.435 7.366 9.217 5.435 7.150 5.968
Pathway and gene set overdispersion analysis